Magoct T, Salzberg SL (2011) FLASH: fast length adjustment of short reads to improve genome assemblies. (Ginkgoaceae) populations from China by RAPD markers. įan XX, Shen L, Zhang X, Chen XY, Fu CX (2004) Assessing genetic diversity of Ginkgo biloba L. Jin YQ, Bi QW, Guan WB, Mao JF (2015) Development of 23 novel polymorphic EST-SSR markers for the endangered relict conifer Metasequoia glyptos troboides. Ĭui MY, Yu S, Liu M, Li YY (2010) Isolation and characterization of polymorphic microsatellite markers in Metasequoia glyptostroboides (Taxodiaceae). Spielman D, Brook BW, Frankham R (2004) Most species are not driven to extinction before genetic factors impact them. įarjon A (2010) A handbook of the world's conifers. Li YY, Ysang EPK, Cui MY, Chen XY (2012) Too early to call it success: an evaluation of the natural regeneration of the endangered Metasequoia glyptostroboides. Santarém F, Pereira P, Saarinen J, Brito JC (2019) New method to identify and map flagship fleets for promoting conservation and ecotourism. ![]() Verissimo D, MacMillan DC, Smith RJ (2011) Toward a systematic approach for identifying conservation flagships. Li YY, Chen XY, Zhang X, Wu TY, Lu HP, Cai YW (2005) Genetic differences between wild and artificial populations of Metasequoia glyptostroboides: implications for species recovery. In: LePage BA, Williams CJ, Yang H (eds) The geobiology and ecology of Metasequoia. LePage BA, Yang H, Matsumoto M (2005) The evolution and biogeographic history of Metasequoia. The novel SSR markers will provide a toolkit for DNA identification of all of the extant wild individuals guiding further conservation efforts of M. ![]() Four to nine loci were cross-amplified successfully in seven species of Cupressaceae. ![]() The observed and expected heterozygosities in each population ranged from 0.0000 to 1.0000 and from 0.0000 to 0.8958, respectively. The number of alleles per locus ranged from 3 to 20, with a mean number of about 8 alleles. Characteristics of each locus were tested using 140 individuals collected from five natural populations of M. We characterized 28 novel polymorphic microsatellite loci using a streptavidin–biotin microsatellite-enriched library and Illumina high-throughput sequencing. glyptostroboides is important to delimit management units and key germplasm resources. Distinguishing the genotypes of all wild individuals of M. Metasequoia glyptostroboides is a living fossil and an endangered species listed in the International Union for Conservation of Nature (IUCN).
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